ENRICHMENT, ISOLATION, AND SCREENING OF DYE-DECOLORIZING HALOTOLERANT MICROBIAL STRAINS

Faramawy, Aya Gamal; Mabrouk, Mona E. M.; Sabry, Soraya A.; Arayes, Mervat Amen

doi:10.21608/jaesj.2023.180885.1048

ENRICHMENT, ISOLATION, AND SCREENING OF DYE-DECOLORIZING HALOTOLERANT MICROBIAL STRAINS

Document Type : Full research articles

Authors

¹ Department of Botany and Microbiology, Faculty of Science, Damanhour University, Egypt

² Department of Botany and Microbiology, Faculty of Science, Alexandria University, Alexandria, Egypt

10.21608/jaesj.2023.180885.1048

Abstract

Recently, the environmental pollution has increased and become one of the serious aspects facing the world due to industrialization and rapid population, this led to the emergence of many problems that have affected the life of living organisms. Among various pollutants are dyes which have multiple industrial uses. Azo dyes are carcinogenic and mutagenic pose hazards to humans, animals and aquatic life. It is more difficult to degrade these dyes because they are recalcitrant. Microbial degradation has been extensively explored. Fungi are an efficient tool in dye decolorization due to their various mechanical features. The purpose of this article is a trial to enrich and isolate different microbial strains inhabit the Egyptian samples and to highlight their potential for remediation by decolorizing dyeing waters. Here, thirteen halotolerant strains were isolated from water and sediment samples of Kom Belal Lake of Idko, Al-Beheira, Egypt, under saline conditions. Out of 13 isolates, eight strains were able to decolorize most of dyes with different degree of removal. Strain A11 was selected due to its high potential to decolorize (congo red, malachite green and methylene blue). The maximum diameter of clear zone created by strain A11 was recorded in the presence of congo red azo dye (35mm). The potent strain A11 which has the highest potential to remove the dye, was morphologically identified as Aspergillus sp. during the screening process. Based on the obtained results presented here, Aspergillus sp. will be candidate for further biotechnological research on the decontamination of dyeing industrial effluents.

Keywords