EFFECT OF THE ADDITION OF PROBIOTICS AS ANTIBIOTIC ALTERNATIVE ON THE QUALITY OF COOLED STORED SEMEN IN RABBITS

Elalfy, Sanaa Salama; Mansour, Mohamed Mohsen; Hashem, Nesrein Mohamed

doi:10.21608/jaesj.2024.244381.1121

EFFECT OF THE ADDITION OF PROBIOTICS AS ANTIBIOTIC ALTERNATIVE ON THE QUALITY OF COOLED STORED SEMEN IN RABBITS

Document Type : Full research articles

Authors

¹ Animal and Poultry Production Department, Faculty of Agriculture, Damanhour University, Egypt.

² Animal and Fish Production Department, Faculty of Agriculture (El-Shatby), Alexandria University, Egypt.

10.21608/jaesj.2024.244381.1121

Abstract

Antibiotics are an essential component of semen extender to avoid a possible depreciation of semen quality. However, antibiotics have been questioned because of an ever-increasing bacterial resistance. In this term, probiotics (Lactococcus lactis) can be used as an alternative to antibiotics due to their antimicrobial properties. In this study, the effects of probiotics (Lactococcus lactis) as an antibiotic alternative compared to control and antibiotic group (gentamicin-GEN) on semen quality in rabbits was studied. The Tris fructose extender was prepared and the supplemented with antibiotic (AB, 250µg gentamycin) probiotic (pro, 10⁹ colony-forming units of Lactococcus lactic) per ml of semen or without supplementation as a control and the semen was cooled at 5^○C for three days. The results showed that probiotics had the same antibiotic-like effect on the quality of cryopreserved semen, except for noticeable differences in sperm motility and gross and vital abnormalities. In conclusion, probiotics (10⁹colony-forming units of Lactococcus lactis) improved semen quality over time thus it could be used as safe alternative to conventional antibiotics in rabbit semen extenders.

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